Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes

Tan, Yi Han (2013) Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes. Masters thesis, Universiti Sains Malaysia.

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Abstract

Six ternary copper(II) complexes of amino acids and polypyridyl ligands with a general formula of [Cu(AA)(B) Cl]·nH2O or [Cu(AA)(B)(H2O)]Cl·nH2O, where AA = DL-threonine, DL-phenylalanine, and DL-valine; B = 2,2’-bipyridyl, 4,4’-dimethyl-2,2’-dipyridyl, and 1,10-phenanthroline are synthesized. The complexes are characterized by CHN elemental analysis, AAS, FT-IR, and UV-Vis and have been structurally characterized by X-ray crystallography. All the complexes formed slightly distorted square-pyramidal coordination geometries. The electronic absorption spectra of the complexes show very low intensity of d-d electronic band in the range of 600-635 nm in Tris-HCl/NaCl (5:5 mM) pH 7.2 buffer solution. The DNA binding interaction with CT-DNA has been investigated by electronic absorption spectral titration and viscosity measurements. The results revealed that the phenanthroline complexes interact with CT-DNA through intercalation while bipyridyl complexes bind to CT-DNA through groove binding mode. The calculated intrinsic binding constant (Kb) of all the complexes are in the range of 0.5-6.2 × 105 M-1. All the copper(II) complexes are found to promote efficient DNA cleavage activities at low concentration in the presence of H2O2. The studies show that [Cu(Phe)(Phen)Cl]·3H2O exhibits the highest DNA binding and nuclease activity among the six copper(II) complexes.

Item Type: Thesis (Masters)
Subjects: T Technology > TP Chemical Technology > TP1-1185 Chemical technology
Divisions: Kampus Kejuruteraan (Engineering Campus) > Pusat Pengajian Kejuruteraan Kimia (School of Chemical Engineering) > Thesis
Depositing User: Mr Mohammad Harish Sabri
Date Deposited: 07 Aug 2019 06:50
Last Modified: 07 Aug 2019 06:50
URI: http://eprints.usm.my/id/eprint/45216

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