Isa, Nur Kamilah Md
(2013)
Solid State Fermentation Of Gibberellic Acid By
Penicillium Variable: Screening, Optimization
And Kinetic Studies.
Masters thesis, Universiti Sains Malaysia.
Abstract
Gibberellic acid (GA3) is one of the family members of gibberellins that have
wider applications toward plant growth acceleration. A normal plant can only
produce growth hormone in a very small quantity, but with sufficient addition of
GA3 could affect the growth development of plant organs.
A few sources of substrates were evaluated for their capabilities as a growth
medium for the production of GA3 in solid state fermentation (SSF). Banana peel
(BP) was found as a suitable substrate as it contain higher amount of carbon and
lower amount of nitrogen source at 44.85 % ± 0.15 and 12.16 % ± 0.16, respectively.
In the present study, sixteen macro- and micro-fungi from different families
(Polyporaceae, Coriolaceae, Lentinaceae, Schizophyllaceae, Trichocomaceae,
Hypocreaceae, and Moniliaceae) were screened for the production of GA3 using BP
as a substrate. It is found that P. variable produced the highest GA3 followed by A.
niger, P. simplicissimum and P. spinulosum at 16.25 ± 0.06 mg/kg substrate, 12.45 ±
0.11 mg/kg substrate, 5.56 ± 0.06 mg/kg substrate and 3.90 ± 0.06 mg/kg substrate,
respectively.
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