Ravinderan, Presela
(2015)
A Novel Nitrocellulose Filter Membrane Based Partitioning-Reverse Transcription Using Repora-6 RNA Aptamer In Detecting Erythropoietin (EPO).
Masters thesis, Universiti Sains Malaysia.
Abstract
Recombinant human EPO (rHuEPO), a mimetic of endogenous EPO is widely misused by athletes as a performance enhancer, a phenomenon known as doping. Current methods of detecting rHuEPO doping are via isoelectric-focusing (IEF)-doubleimmunoblotting, Sarcosyl-PAGE (SAR PAGE) and lateral flow test, which are antibody-based approach using anti-EPO antibody. The caveats associated with anti- EPO antibodies have spurred the interest to adopt an alternative molecular recognition element (MRE) in detecting EPO.
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