Ishak, Rafidah
(2012)
Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids.
Masters thesis, Universiti Sains Malaysia.
Abstract
Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be induced from the leaf, petiole and root explants of the in vitro plantlets on MS medium supplemented with 2.0 - 10.0 mg/L picloram. The leaf explants inoculated to MS medium supplemented with 2.0 mg/L picloram produced the best callus for cell suspension cultures.
Actions (login required)
|
View Item |