Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28

Julkifle, Advina Lizah (2012) Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28. Masters thesis, Universiti Sains Malaysia.

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Abstract

Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid and liquid cultures. In sucrose treatment, the highest PLBs growth rate percentage was in 10 g.L-1 sucrose. In the conditions of absence of sucrose (0 g.L-1) or excessive of sucrose (30 g.L-1) had proven detrimental for the PLBs growth and development.

Item Type: Thesis (Masters)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: ASM Ab Shukor Mustapa
Date Deposited: 01 Jul 2019 02:11
Last Modified: 01 Jul 2019 02:11
URI: http://eprints.usm.my/id/eprint/44776

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