Ismail, Norzila and Ibrahim, Wan Suriyani Wan and Salleh, Siti Farhanah Mohd and Yajid, Aidy Irman and Wong, Michael Pak-Kai and Hashim, Mohd Nizam Md (2019) Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G0/G1 and upregulating Bax. Asian Pacific Journal of Tropical Biomedicine, 9 (6). pp. 249-256. ISSN 2221-1691 (Submitted)
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Abstract
Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf extracts and their effect on cell death. Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate and methanol by Soxhlet extraction. Aqueous extract was prepared by decoction at 50. Extracts of A. precatorius leaves were used to treat selected cancer and normal cell lines for 72 h. Furthermore, 3-(4,5-dimethyl thiazol-2-yl) 2,5-diphenyl tetrazolium bromide assay was performed to determine cell viability. Analysis of cell cycle arrest, apoptosis assay and apoptosis protein expressions were determined by flow cytometry. Results: Methanolic extract of A. precatorius leaves showed the lowest IC50 on MDA-MB-231 cells at (26.40依5.40) μg/mL. Flow cytometry analysis revealed that cell arrest occurred at G0/ G1 phase and the apoptosis assay showed the occurrence of early apoptosis at 48 h in MDAMB- 231 cells treated with methanolic extract of A. precatorius leaves. Methanolic extract of A. precatorius leaves induced apoptosis by upregulation of Bax, p53 and caspase-3 and downregulation of Bcl-2. Conclusions: Methanolic extract of A. precatorius leaves promotes MDA-MB-231 cell death by inducing cell cycle arrest and apoptosis possibly via the mitochondrial-related pathway.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cancer |
| Subjects: | R Medicine > RC Internal medicine > RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| Divisions: | Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Article Zoom Profil Pakar (Expert Profile) > Norzila Ismail (Medical Sciences) |
| Depositing User: | Mr Abdul Hadi Mohammad |
| Date Deposited: | 20 Jun 2019 08:11 |
| Last Modified: | 27 Feb 2020 08:17 |
| URI: | http://eprints.usm.my/id/eprint/44687 |
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