Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules

Khoo, Miew Leng (2018) Anti-Candidal Activity And Toxicity Studies Of Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Fe-Bovine Lactoferrin Nanocapsules. PhD thesis, Universiti Sains Malaysia.

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Abstract

Lactoferrin has been known to have antimicrobial properties. This research was conducted to investigate the anti-candidal activity and toxicity of Alginate/EUDRAGIT® S 100-enclosed chitosan–calcium phosphate-loaded Fe-bLf nanocapsules (NCs) by in vitro and in vivo study. The screening of NCs for antimicrobial activity showed that NCs has good anti-candidal activity. The minimum inhibitory concentration (MIC) value of NCs using broth microdilution method was found to be 500 μg/mL. The growth profile study demonstrated that the NCs at MIC, two times MIC and four times MIC inhibited the growth of Candida albicans USM-K1 (C. albicans). The electron microscopy studies showed that NCs caused the destruction of yeast cells by disruption of cell wall and cell membrane with increase of incubation time until 36 hours.

Item Type: Thesis (PhD)
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions: Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Thesis
Depositing User: ASM Ab Shukor Mustapa
Date Deposited: 11 Apr 2019 01:40
Last Modified: 12 Apr 2019 05:24
URI: http://eprints.usm.my/id/eprint/44020

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