Zainal, Tengku Siti Nor Zaimas Tengku
(2012)
Potential Of Endophytic Bacteria From Papaya (Carica Papaya) To Control Colletotrichum Gloeosporioides.
Masters thesis, Universiti Sains Malaysia.
Abstract
Current approaches to control papaya disease rely on the use of chemicals
fungicide which is detrimental to human health and environment. Thus, there is a need to
find and develop biological means of controlling pathogens of papaya. One of the most
serious threats to papaya crop is anthracnose disease caused by Colletotrichum
gloeosporioides. This study was conducted to investigate the potential of endophytic
bacteria from Carica papaya as biological control agents against anthracnose by in vitro
means. A total of 47 and 57 bacterial endophytes were isolated from fruits and leaves of
papaya, respectively. The endophytic bacteria were identified based on their
morphological and physiological characteristics, BIOLOG Identification System, and
16S rDNA sequence analysis. Based on the results, both strains F21 and L63 were
identified as Bacillus species. These Bacillus spp. successfully showed inhibitory effects
against C. gloeosporioides isolates 1 and 2. Based on dual culture test, Bacillus sp. L63
has the potential to be biocontrol agent against C. gloeosporiroides isolates 1 and 2 with
percentage of inhibition 47.05% and 58.06%, respectively. Severe morphological
changes were observed on the fungal mycelia when treated with Bacillus sp. L63. In
conclusion, Bacillus sp. L63 has the potential to serve as biological control agents
against C. gloeosporioides.
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