Ishak, Mohd Nurmuhaimin Adli
(2013)
Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia.
Masters thesis, Universiti Sains Malaysia.
Abstract
In Malaysia, tobacco industry is very crucial in uplifting the socio-economic status
of farmer in Kelantan and Terengganu. Root-knot nematodes (Meloidogyne spp.)
lower the production and quality of tobacco. In this study, 24 tobaccos cultivation
areas were surveyed for root gall disease and species diversity of root- knot
nematode. Soil physical and pH analysis were carried out to investigate their
correlation to disease severity and number of root-knot nematode number in soil.
Isolated Meloidogyne spp. was observed for morphology for genus identification and
further analyses via Touchdown Polymerase chain reaction (TD-PCR) for species
identification. From 24 cultivation area, 22 showed infestation of root-knot
nematode. Tobacco plant infected with root-knot nematode showed root gall,
yellowing leaves and stunted growth. One- Way ANOVA analysis showed that soil
physical properties, and soil pH affect disease severity and Meloidogyne spp. number
in soil. Meloidogyne spp. numbers in soil also affect disease severity. From Pearson
correlation analysis, there was significant correlation at 0.01 between Meloidogyne
spp. number in soil (r = 0.753), soil pH (r = 0.238), soil moisture (r = 0.203) and soil
bulk density (r = 0.227) to disease severity.
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