Razak, Mohd Shahril Firdaus Ab
(2012)
A Genetic Linkage Map Of Asian Seabass,
Lates Calcarifer Using Dna Microsatellite
Markers In Application To Quantitative
Trait Loci (Qtl).
Masters thesis, Universiti Sains Malaysia.
Abstract
Genetic linkage maps of Asian sea bass have been established using a
mapping panel containing a pair of parent (1 male and 1 female) and their 160
progenies. This mapping panel was obtained from parentage assignment from mass
breeding programme using DNA microsatellite markers. In parentage assignment, a
total 24 pairs of microsatellite markers have been used to screen all broodstock. The
analyses result showed the number of alleles per locus ranged from 3 to 14 with an
average of 6.65 alleles per locus and the values of the observed and expected
heterozygosities ranged from 0.121 to 1.000 and from 0.198 to 0.857 respectively.
The average of PIC was 0.515 with range from 0.186 to 0.826. From this analysis,
only five microsatellite markers were used in parentage assignment since these 5
markers show high polymorphism level. In parentage analyses, 94.9% were
successfully assigned to a single or double parent pair and only 51 (5.1%) individual
progenies were left unassigned since these individual were genotyped for fewer than
2 loci. A single parent pair with their progeny have been selected and used as a
mapping panel in linkage mapping construction. In linkage analyses, a total of 273
microsatellite markers have been used. Out of 273 markers, only 210 microsatellite
markers (76.9%) were successfully mapped into 24 linkage groups (labelled LG1-
LG24) at minimum 3.0 LOD value and maximum distance at 50.0 cM
(centiMorgan). The rest of the markers were not mapped in our analysis because they
showed the presence of null alleles, unlinked markers and monomorphic
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