Yeoh, Lay Chin
(2010)
Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients.
Masters thesis, Universiti Sains Malaysia.
Abstract
Colorectal cancer (CRC) is on the rise. However, biomarkers for
indication of CRC progression is still lacking despite several advances in
proteomic and microarray tools. This study aims to identify potential biomarkers
for the diagnosis of CRC among Malaysian patients. In this study, sequential
extraction was first performed using Tris buffer followed by TLB buffer in order
to reduce the complexity of the CRC proteome. The tissues used were surgical
resections of normal mucosa and cancerous tissues from 26 CRC patients. The
extracted proteins were separated by 2D gel electrophoresis. The differentially
expressed protein spots were then mapped for further protein identification by the
use of liquid chromatography tandem mass spectrometry (LC-MS/MS). We have
identified 37 hydrophilic proteins in the Tris extracts and 24 hydrophobic
proteins in the thiourea lysis buffer (TLB) extracts from the samples collected.
Leukocyte elastase inhibitor (LEI) and 60 kDa heat shock protein (HSP60) were
the potential biomarkers for CRC analyzed from the Tris extracts whereas
triosephosphate isomerase (TPI) and annexin A3 were the potential biomarkers for CRC analyzed from the TLB extracts. Moreover, LEI was the potential
biomarker for CRC patients at stage III in combination with either moderately
differentiated adenocarcinoma (MDA), well differentiated adenocarcinoma
(WDA) or lymph node metastasis CRC.
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