Construction Of Vectors For The Overexpression Of Recombinant Human Growth Hormone In Bacillus Megaterium

Rashid, Norhashima Abd. (2009) Construction Of Vectors For The Overexpression Of Recombinant Human Growth Hormone In Bacillus Megaterium. Masters thesis, Universiti Sains Malaysia.

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Abstract

Bagi mengekspreskan gen hGH di dalam Bacillus megaterium, 3 konstrak telah direka. Konstrak-konstrak ini dinamakan sebagai Construct 1 (M7hGH), Construct 2 (R2L4sphGH) dan Construct 3 (spBsubhGH). Construct 1 direka dengan memasukkan gen pengawalatur (kodon penamat, tapak perlekatan ribosom, jujukan TAACA dan kodon pemula) yang akan diekspreskan di dalam sel. Manakala Construct 2 telah dicipta untuk diekpreskan di luar sel yang mana mengandungi isyarat peptida daripada Bacillus brevis dan gen. In order to express the hGH gene in Bacillus megaterium, 3 constructs were designed. The constructs were named as Construct 1 (M7hGH), Construct 2 (R2L4sphGH) and Construct 3 (spBsubhGH). Construct 1 was designed with regulatory features [stop codon, ribosome binding site (RBS), TAACA sequence and start codon] meant for intracellular expression. Construct 2, was created to express human growth hormone (hGH) extracellularly by using the signal peptide from Bacillus brevis.

Item Type: Thesis (Masters)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: Mr Mohammad Harish Sabri
Date Deposited: 13 Aug 2018 06:50
Last Modified: 12 Apr 2019 05:27
URI: http://eprints.usm.my/id/eprint/41298

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