Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum

Zulkipli, Anis Fadhilah (2016) Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum. Masters thesis, USM.

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Abstract

Apoptosis makrofaj merupakan mekanisme yang berkesan dalam mengawal jangkitan intrasel semasa gerak balas imun semulajadi terhadap pelbagai patogen termasuk parasit malaria. Kajian ini dijalankan untuk menentukan aktiviti apoptosis dan pengekspresan protein pengaktifan mitogen (MAPK) dalam sel makrofaj mencit J774A.1 yang dijangkiti klon BCG dan BCG rekombinan (rBCG) yang mengekspreskan terminus C protein permukaan merozoite-1 (MSP-1C) daripada Plasmodium falciparum selama 48 jam. Pewarnaan nukleus menggunakan Hoest 33342 menunjukkan bahawa klon rBCG berupaya meningkatkan kondensasi nuklear dan peringkat morfologi apoptosis dalam sel makrofaj yang dijangkiti berbanding BCG dan LPS. Macrophage apoptosis exerts an efficient mechanism in controlling intracellular infection during innate immune response against various pathogens including malaria parasites. This study was carried out to determine the apoptosis activity and mitogen activated protein kinase (MAPK) expression in mouse macrophage cell line J774A.1 infected with a Mycobacterium bovis bacille Calmette-Guerin (BCG) clone and a recombinant BCG (rBCG) clone expressing the C-terminus of merozoite surface protein-1 (MSP-1C) of Plasmodium falciparum for 48 hours. The nuclear staining with Hoechst 33342 showed that the rBCG clone was capable of increasing the nuclear condensation and morphological stages of apoptosis in the infected cells compared to the BCG-infected cells and the LPS-stimulated cells. The flow cytometric analysis using Annexin-V and PI staining confirmed that the rBCG clone significantly increased the percentage of early apoptotic activity in the infected macrophage higher than the one stimulated by the parent BCG clone and LPS.

Item Type: Thesis (Masters)
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Thesis
Depositing User: ARKM Al Rashid Automasi
Date Deposited: 28 Jun 2018 08:19
Last Modified: 28 Jun 2018 08:19
URI: http://eprints.usm.my/id/eprint/40846

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