Vigneswari, S. and Lee, T. S. and Bhubalan, Kesaven and Amirul, A. A.
(2015)
Extracellular Polyhydroxyalkanoate Depolymerase by Acidovorax sp. DP5.
Enzyme Research, 2015 (212159).
pp. 1-8.
ISSN 2090-0406
Abstract
Bacteria capable of degrading polyhydroxyalkanoates (PHA) by secreting extracellular depolymerase enzymes were isolated from
water and soil samples collected fromvarious environments in Malaysia. A total of 8 potential degraders exhibited clear zones on
poly(3-hydroxybutyrate) [P(3HB)] based agar, indicating the presence of extracellular PHAdepolymerase.Among the isolates,DP5
exhibited the largest clearing zone with a degradation index of 6.0. The highest degradation activity of P(3HB) was also observed
with depolymerase enzyme of DP5 in mineral salt medium containing P(3HB). Based on biochemical characterization and 16S
rRNA cloning and sequencing, isolate DP5 was found to belong to the genus Acidovorax and subsequently named as Acidovorax
sp. DP5.The highest extracellular depolymerase enzyme activity was achieved when 0.25% (w/v) of P(3HB) and 1 g/L of urea were
used as carbon and nitrogen source, respectively, in the culture media. The most suitable assay condition of the depolymerase
enzyme in response to pH and temperature was tested. The depolymerase produced by strain Acidovorax sp. DP5 showed high
percentage of degradation with P(3HB) films in an alkaline condition with pH 9 and at a temperature of 40∘C.
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