Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies

Nguyen Thi, Le Thuy and Maura, Reinier Borrero and Férnandez, Sonsire and Reyes, Giselle and Perez, José Luis and Reyes, Fátima and García, María de los Angeles and Fariñas, Midrey and Infante, Juan Francisco and Tirado, Yanely and Puig, Alina and Sierra, Gustavo and Álvarez, Nadine and Ramírez, Juan Carlos and Sarmiento, María Elena and Norazmi, Mohd Nor and Acosta, Armando (2010) Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies. VacciMonitor, 19 (1). pp. 20-26. ISSN 1025-028X

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Abstract

In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent and reactivation phases. The selected proteins were predicted for T and B epitopes. For each proteins, the regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis based on sequence alignments. Preliminary studies of humoral immunogenicity and cross-reactivity with M. tuberculosis in mice using two M. smegmatis-derived experimental vaccines were carried out, demonstrating the immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of animals immunized with this vaccine candidate. The conjunction of in silico and in vivo studies suggested the potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies

Item Type: Article
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Article
Depositing User: Mr Noorazilan Noordin
Date Deposited: 20 Feb 2018 01:41
Last Modified: 20 Feb 2018 01:41
URI: http://eprints.usm.my/id/eprint/39018

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