Nguyen Thi, Le Thuy and Maura, Reinier Borrero and Férnandez, Sonsire and Reyes, Giselle and Perez, José Luis and Reyes, Fátima and García, María de los Angeles and Fariñas, Midrey and Infante, Juan Francisco and Tirado, Yanely and Puig, Alina and Sierra, Gustavo and Álvarez, Nadine and Ramírez, Juan Carlos and Sarmiento, María Elena and Norazmi, Mohd Nor and Acosta, Armando
(2010)
Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies.
VacciMonitor, 19 (1).
pp. 20-26.
ISSN 1025-028X
Abstract
In this study, we scanned multiple published databases of gene expression in vivo of M. tuberculosis at different
phases of infection in animals and humans, to select 38 proteins that are highly expressed in the active, latent
and reactivation phases. The selected proteins were predicted for T and B epitopes. For each proteins, the
regions containing T and B epitopes were selected at the same time to look for identical epitopes on M. smegmatis
based on sequence alignments. Preliminary studies of humoral immunogenicity and cross-reactivity with M.
tuberculosis in mice using two M. smegmatis-derived experimental vaccines were carried out, demonstrating the
immunogenicity of M. smegmatis proteoliposomes and the recognition of M. tuberculosis proteins by the sera of
animals immunized with this vaccine candidate. The conjunction of in silico and in vivo studies suggested the
potential for future evaluation of M. smegmatis as vaccine candidate against tuberculosis using different strategies
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