Antony, Jessica Jeyanthi James and Leong, Kah Wai and Oyunbileg, Yungeree and Subramaniam, Sreeramanan
(2015)
Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses.
Mongolian Journal of Biological Sciences, 13 (1-2).
pp. 13-18.
ISSN 1684-3908
Abstract
This study was conducted to detect the morphological, histological and molecular
diff erences in the second generation of the PVS2 cryopreserved Dendrobium
Bobby Messina [DBM] (18 months old culture) plantlets. Morphological analyses
indicated that similarities and diff erences in cryopreserved DBM plantlets comparing
to control stock culture based on selected morphological criteria. Morphological
criteria, such as root length, number of shoot per explant and shoot length displayed
diff erences, while the other three criteria, leaf diameter, leaf length and PLBs size
were similar in cryopreserved compared to the control stock culture plant. Higher
amount of homogenous cell population and denser cytoplasm were observed in
cryopreserved PLBs compared to control stock culture PLBs based on histological
analysis. This suggests the existance of somatic embryogenesis development
mechanism taking place during the recovery and regeneration of the cryopreserved
PLBs. However, RAPD analyses based on 10 primers indicated that cryopreserved
DBM regenerated from vitrifi cation method generated a total of 20 to 39.9%
polymorphic bands as compared to stock culture indicating potential somaclonal
variation. Hence, an increase percentage of polymorphics bands in cryopreserved
plantlets 18 months post cryopreservation as compared to previous report of 10%
polymorphic bands in cryopreserved DBM 3 months post cryopreservation.
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