Khalil, Md. Ibrahim and Ahmmed, Istiyak and Ahmed, Romana and E. M., Tanvir and Afroz, Rizwana and Paul, Sudip and Siew, Hua Gan and Alam, Nadia
(2015)
Amelioration of Isoproterenol-Induced Oxidative Damage in Rat
Myocardium by Withania somnifera Leaf Extract.
BioMed Research International, 2015 (624159).
pp. 1-10.
ISSN 2314-6133
Abstract
We investigated the protective role of Withania somnifera leaf extract (WSLEt) on isoproterenol- (ISO-) induced myocardial
infarction (MI) in rats. Subcutaneous injection of ISO (85mg/kg body weight (b.w.)) administered to rats for two consecutive days
caused a significant increase in cardiac troponin I (cTnI) levels and serum lipid profiles, as well as the activities of some marker
enzymes. In addition to these diagnosticmarkers, there were increased levels of lipid peroxidation (LPO) and decreased activities of
enzymatic antioxidants (superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRx), and glutathione-
S-transferase (GST)) in themyocardium.However, oral pretreatment (100mg/kg b.w.) withWSLEt for 4 weeks elicited a significant
cardioprotective activity by lowering the levels of cTnI, lipid profiles, and marker enzymes. The levels of LPO products were also
significantly decreased. Elevated activities of antioxidant enzymes were also observed in rats pretreated with WSLEt. As further
confirmed histopathologically, our findings strongly suggest that the cardioprotective effect ofWSLEt onmyocardium experiencing
ISO-induced oxidative damage may be due to an augmentation of the endogenous antioxidant system and an inhibition of LPO in
the myocardial membrane.We conclude thatWSLEt confers some protection against oxidative damage in ISO-inducedMI in rats.
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