Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency

Gnasekaran, Pavallekoodi and Antony, Jessica Jeyanthi James and Mohammad, Jasim Uddain and Subramaniam, Sreeramanan (2014) Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency. Scientific World Journal, 2014 (583934). pp. 1-10. ISSN 2356-6140

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Abstract

The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem’s Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and quantified using gusAgene expression to optimize the efficiency of Agrobacterium-mediated genetic transformation of VKD’s PLBs. Based on the results, 3-4mm PLBs wounded by scalpel and immersed for 30 minutes in Agrobacterium suspension of 0.8 unit at

Item Type:	Article
Subjects:	Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions:	Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Article Zoom Profil Pakar (Expert Profile) > Sreeramanan A/L Subramaniam (Biological Sciences)
Depositing User:	Mr Noorazilan Noordin
Date Deposited:	08 Jan 2018 01:53
Last Modified:	06 May 2020 06:46
URI:	http://eprints.usm.my/id/eprint/38273

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