Saniasiaya, Jeyasakthy and Salim, Rosdan and Mohamad, Irfan and Harun, Azian
(2017)
Antifungal Effect of Malaysian Aloe vera Leaf
Extract on Selected Fungal Species of Pathogenic
Otomycosis Species in In Vitro Culture Medium.
Oman Medical Journal, 32 (1).
pp. 41-46.
ISSN 1999-768X
Abstract
Objectives: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes
since ancient times with antifungal activity known to be amongst its medicinal properties.
We conducted a pilot study to determine the antifungal properties of Malaysian Aloe
vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans.
Methods: This laboratory-controlled prospective study was conducted at the Universiti
Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions
via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal
isolates were inoculated with the five different concentrations of each extract (50 g/mL,
25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method.
Zone of inhibition was measured followed by minimum inhibitory concentration
(MIC). Results: For A. niger, a zone of inhibition for alcohol and aqueous extract
was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for
both alcohol and aqueous extracts of Aloe vera leaf for C. albicans. The MIC values of
aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone
of inhibition was obtained for C. albicans the MIC was not determined. Conclusions:
The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the
aqueous extract for A. niger (p < 0.001). Malaysian Aloe vera has a significant antifungal
effect towards A. niger
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