Mohd Noor, Juliana
(2011)
Determination Of Selected Organochlorine Pesticides In Farmers' Biological Samples Using Gas Chromatography-Mass Spectrometry And Their Knowledge, Attitude And Practice Towards Pesticides Use.
Masters thesis, Universiti Sains Malaysia.
Abstract
Kajian ini dilakukan di kawasan pertanian di Pinang Tunggal, Kepala Batas, Pulau
Pinang. Tujuan utama kajian ini ialah untuk menentukan paras racun makhluk perosak
organoklorin terpilih dalam sampel-sampel biologi petani terutama rambut dan urin
dengan menggunakan Kromatografi Gas - Spektrometri Jisim. Seiring dengan itu, ia
turut dilakukan bagi menilai pengetahuan, tanggapan dan amalan petani terhadap
penggunaan racun makhluk perosak. Organoklorin terpilih dalam kajian ini ialah HCHs
(a-, b-, y- dan 8-hch), DDTs (p ,p'-ddt, p ,p'-ddd dan p,p'-dde), aldrin, dieldrin,
endosulfan sulfate, heptaklor, heptaklor epoksid dan metoksiklor. Sampel rambut dipilih
kerana diketahui ia sebagai penunjuk pendedahan jangka masa panjang yang baik
terhadap pendedahan bahan kimia manakala urin biasanya digunakan untuk menentukan
pendedahan jangka masa pendek. Pembangunan dan pengesahan kaedah telah disahkan
dan beberapa parameter telah dianalisis. Parameter yang diperhatikan adalah masa dan
kelajuan untuk penyebatian, kelajuan dan masa untuk pengemparan, suhu untuk
penghancuran rambut dan pelarut untuk pengekstrakan. R2 berada dalam julat yang
diterima, pekali-pekali variasi adalah kurang daripada 20% dan pemerolehan semula
berada dalam lingkungan 70 - 120%. 33 % sampel rambut (n=60) dan 21% .contoh urin
(n=60) daripada responden telah dikesan mengandungi kepekatan tertentu beberapa
racun perosak terpilih itu.
This study was conducted in agricultural areas in Pinang Tunggal, Kepala Batas,
Penang. The main objective of the study was to determine the level of selected
organochlorine pesticides in farmers' biological samples particularly in hair and urine
samples by using Gas Chromatography- Mass Spectrometry (GC-MS). Concomitantly,
it was also carried out to assess the farmers' knowledge, attitude and practice towards
pesticide use. Organochlorine pesticides selected in this study were HCHs (a-, b-, y- and
8-hch), DDTs (p,p'-ddt, p,p'-ddd and p,p'-dde), aldrin, dieldrin, endosulfan sulfate,
heptachlor, heptachlor epoxide and methoxychlor. Hair sample was chosen as it is
known to be a good long term indicator for chemical exposure while urine is normally
used to determine short term exposure. Method development and validation using GCMS
were established and several parameters were analyzed. Parameters observed were
speed and time for agitation, speed and time for centrifugation, temperature for hair
digestion and type of solvent for extraction. R2 was in accepted range, coefficients of
variation were less than 20% and recoveries were in the range of 70% to 120%. A total
33 % of the hair ·samples (n=60) and 21% of the urine samples (n=60) from the
respondents were detected to contain certain concentrations of the pesticides.
Actions (login required)
 |
View Item |
![[img]](http://eprints.usm.my/style/images/fileicons/application_pdf.png)
