Jamaludin, Nur Liyana
(2016)
Anti-Mycobacterial Activity Of Labisia Pumila Benth. & Hook. F. And Its Bioactive Constituents Against Surrogate Tuberculosis Organisms.
Masters thesis, Universiti Sains Malaysia.
Abstract
Kemunculan strain tuberkulosis (TB) rintang pelbagai drug menjadi faktor
utama yang menyumbang kepada peningkatan jangkitan TB secara global. Oleh yang
demikian, keperluan yang mendesak bagi drug anti-TB yang efektif, selamat dan
murah telah mendorong banyak kajian dijalankan terhadap produk semulajadi
termasuk tumbuhan. Bahagian daripada Labisia pumila telah dikaji untuk mengenal
pasti potensi anti-mikobakterianya terhadap organisma pengganti
Mycobacterium tuberculosis (M. smegmatis, M. fortuitum dan M. kansasii)
menggunakan asai pencairanmikro tetrazolium. Bahagian paling aktif telah
dihasilkan oleh n-heksana batang-akar dan daun terhadap M. kansasii dengan
kepekatan perencat minima (MIC) 50 - 200 μg/mL. Bahagian ini telah dipilih untuk
proses fraksinasi berpandukan bioasai menggunakan teknik kromatografi turus yang
telah menghasilkan 20 jenis fraksi yang berbeza. Fraksi ini telah diuji terhadap
M. kansasii dan fraksi R2, R6 dan L7 telah mempamerkan aktiviti paling
memberangsangkan (MIC: 25 - 50 μg/mL). Ujian saringan lanjutan terhadap fraksi
aktif ini telah dijalankan terhadap strain M. tuberculosis H37Ra dan nilai MIC yang
dihasilkan masing – masing adalah 12.5, 50 dan 100 μg/mL.
The development of multidrug resistant tuberculosis (TB) strains becomes a
major factor contributing to the rise of global TB incidence. Therefore, the urgent
need of effective, safe and inexpensive anti-TB drugs has geared many researches
towards natural products including plants. Partitions from Labisia pumila were
investigated to evaluate their anti-mycobacterial potential against Mycobacterium
tuberculosis surrogate organisms (M. smegmatis, M. fortuitum, and M. kansasii)
using tetrazolium microdilution assay. The most active partitions were produced by
n-hexane of stem-root and leaf parts against M. kansasii with minimum inhibitory
concentrations (MICs) of 50 - 200 μg/mL. These partitions were selected for
bioassay guided fractionation process using column chromatographic techniques,
which produced 20 different fractions. These fractions were screened against
M. kansasii and fractions R2, R6, and L7 exhibited the most promising activity
(MIC: 25 – 50 μg/mL). Further screening of these active fractions was carried out
against M. tuberculosis H37Ra strain and the MIC values produced were 12.5, 50
and 100, μg/mL, respectively.
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