Othman, Ekhlas Qaid Gazem
(2016)
Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue.
PhD thesis, Universiti Sains Malaysia.
Abstract
Pengesanan beberapa jenis kanser boleh dilakukan dengan menggunakan
antibodi monoklon (AbM). Kajian menunjukkan bahawa jenis-jenis kanser yang
berbeza mempunyai protein tak normal yang tertentu yang boleh dikenal pasti oleh
AbM. IBMR3 AbM dibangunkan daripada peptida sintetik yang berhubung dengan
jujukan asid amino terpilih daripada reseptor interleukin-4 manusia. Kajian ini adalah
untuk mengesan ekspresi IBMR3 antigen dalam karsinoma termasuk Manusia
carcinoma berhubung dgn pangkal tekak epitelium (HEp-2), adenokarsinoma kolon
Manusia (HT-29) dan tisu kanser payudara. Ungkapan IBMR3 antigen dikesan pada
HEp-2 dan HT-29 menggunakan ujian berbentuk tidak langsung pewarnaan, aliran
sitometri pewarnaan. Hasil kajian menunjukkan persatuan kemungkinan IBMR3
antigen ungkapan dalam karsinoma (kanser sel-sel epitelium) dengan pewarnaan
cytoplasmic positif. HEp-2 dan HT-29 telah dipisahkan melalui 1-D immunoblot dan
keputusan menunjukkan dua band dengan berat molekul (MWS) 45 dan 25 kDa
dalam HT-29 dan satu band dengan MWS 25 kDa dalam HEp-2. IBMR3 antigen
ungkapan itu dikesan pada 48 sampel kanser payudara Malaysia dan microarray
(TMA) slaid tisu kanser payudara 430 dan 132 tisu payudara yang normal
bersebelahan dengan kanser payudara melalui IHC.
The detection of several types of cancer is possible by using monoclonal
antibody (MAb). Studies have shown that different types of cancer have related
specific abnormal proteins, which are identified by MAb. IBMR3 is a MAb which
was raised against synthetic peptides that are associated with the selected amino acid
sequence of human interleukin-4 receptors. This research is to detect the expression
of IBMR3 antigen in carcinoma including Human laryngeal epithelial carcinoma
(HEp-2), Human colon adenocarcinoma (HT-29) and breast cancer tissues. The
expression of IBMR3 antigen was detected on HEp-2 and HT-29 using indirect
immunofluorescent staining, flow cytometry staining. The results showed the
possible association of IBMR3 antigen expression in carcinoma (epithelial cancer
cells) with positive cytoplasmic staining. HEp-2 and HT-29 were separated via 1-D
immunoblot and the results showed 2 bands with molecular weights (MWs) of 45
and 25 kDa in HT-29 and one band with MWs of 25 kDa in HEp-2. IBMR3 antigen
expression was detected on 48 samples of Malaysian breast cancer and microarray
(TMA) slides of 430 breast cancer tissues and 132 normal breast tissues adjacent to
breast cancer through IHC.
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