Jamaludin, Azirah Inez
(2016)
In Vitro Anti-Mycobacterial And Bioactive Compounds Of Pluchea Indica (L.) Less. Against Surrogate Tuberculosis Organisms.
Masters thesis, Universiti Sains Malaysia.
Abstract
Pluchea indica (L.) Less. merupakan tumbuhan ubatan Asia yang digunakan
secara meluas dalam perubatan tradisional untuk merawat pelbagai penyakit termasuk
gejala tuberkulosis (TB) dan oleh itu, dapat menyediakan sumber alternatif sebatian
anti-TB. Penyaringan awal in vitro tiga bahagian berbeza P. indica menggunakan asai
pencairanmikro tetrazolium menunjukkan bahagian n-heksana daun dan batang dan
kloroform daun mempamerkan aktiviti rencatan terhadap Mycobacterium smegmatis,
M. fortuitum and M. kansasii dengan kepekatan perencat minimum (MIC) berjulat
antara 200 - 3200 μg/mL. Aktiviti paling tinggi ditunjukkan oleh n-heksana daun
terhadap M. kansasii dengan MIC 200 μg/mL. Pemecahan berpandu bioasai bahagian
n-heksana daun dilakukan untuk analisis selanjutnya menggunakan kromatografi
lapisan nipis dan kromatografi turus yang menghasilkan lapan fraksi (Fraksi A - H).
Fraksi tersebut mempamerkan aktiviti terhadap M. kansasii dengan MIC berjulat
antara 12.5 - 800 μg/mL. Fraksi yang sangat aktif (Fraksi A, C, D, dan E) juga
merencat M. tuberculosis H37Ra dengan MIC berjulat antara 50 - 100 μg/mL.
Pengenalpastian fitokimia fraksi paling aktif, Fraksi C menggunakan gas
kromatografi-spektrometri jisim menghasilkan 10 sebatian yang dikenal pasti, yang
mana asid n-heksadekanoik, asid 9,12-oktadekadienoik (Z,Z)-, dan asid oktadekanoik
adalah sebatian utama.
Pluchea indica (L.) Less. has been used widely in traditional medicine as a
remedy of a variety of illnesses including symptoms of tuberculosis (TB) and thus,
could provide an alternative source of anti-TB compounds. Preliminary in vitro
screening of three different P. indica partitions, using tetrazolium microdilution assay
showed that leaf and stem n-hexane and leaf chloroform partitions exhibited inhibitory
activity against Mycobacterium smegmatis, M. fortuitum and M. kansasii with
minimum inhibitory concentrations (MICs) of 200 - 3200 μg/mL. The highest activity
was shown by leaf n-hexane against M. kansasii with MIC of 200 μg/mL. The leaf
n-hexane partition was selected for further bioassay guided fractionation process using
thin layer and column chromatographic techniques, which yielded eight fractions
(Fractions A - H). These fractions exhibited activity against M. kansasii with MICs in
the range of 12.5 – 800 μg/mL. The highly active fractions (A, C, D, and E) also
inhibited M. tuberculosis H37Ra with MICs in the range of 50 – 100 μg/mL.
Phytochemical identifications of the most active fraction, Fraction C using gas
chromatography-mass spectrometry produced 10 identified compounds, of which,
n-hexadecanoic acid, 9,12-octadecadienoic acid (Z,Z)-, and octadecanoic acid were
the major compounds.
Actions (login required)
 |
View Item |
![[img]](http://eprints.usm.my/style/images/fileicons/application_pdf.png)
