Lim, Ying Yu
(2015)
Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants.
Masters thesis, Universiti Sains Malaysia.
Abstract
Ciri-ciri anti-penuaan ekstrak daripada empat jenis tumbuh-tumbuhan yang berbeza,
iaitu O. stamineus, C. hirta, P. sarmentosum dan C. caudatus telah disiasat. Kaedah
pengekstrakan yang mudah telah dijalankan sebelum analisis yang seterusnya. Asai
DPPH menunjukkan bahawa ekstrak etanol C. hirta menunjukkan aktiviti
memerangkap radikal yang tertinggi, dengan IC50 33.3±1.8μg/ml manakala ekstrak
etanol P. sarmentosum menunjukkan aktiviti memerangkap radikal yang terendah,
dengan IC50 660.4±14.4μg/ml. Toksisiti ekstrak tumbuh-tumbuhan telah diuji dengan
menggunakan sel-sel fibroblast kulit manusia dewasa. O. stamineus dan C. caudatus
adalah toksik kepada sel-sel pada kepekatan 100μg/ml. Kesan-kesan daripada ekstrak
tumbuh-tumbuhan terhadap prokolagen I telah diuji dan didapati bahawa O.
stamineus, C. hirta dan C. caudatus meningkatkan ekspresi PICP apabila diuji pada
kepekatan 50, 100, 250 dan 500ng/ml. Untuk kesan perlindungan ekstrak tumbuhtumbuhan
pada kepekatan 50, 100, 250 dan 500ng/ml terhadap dos sublethal
hidrogen peroksida pada 750μg/ml, hanya 50ng/ml O. stamineus menunjukkan
perlindungan terhadap tekanan oksidatif. Ekstrak tumbuh-tumbuhan telah disiasat
selanjutnya dengan menggunakan C. elegans sebagai model organisma in vivo.
The anti-aging properties of extracts from four different plants, namely O. stamineus,
C. hirta, P. sarmentosum and C. caudatus were investigated. A simple extraction
method was carried out before further analysis. DPPH assay indicated that ethanol
extract of C. hirta showed the highest radical scavenging activities, with IC50
of 33.3
± 1.8 μg/ml whilst ethanol extract of P. sarmentosum showed the lowest radical
scavenging activities, with IC50 of 660.4 ± 14.4 μg/ml. Toxicity of plant extracts was
tested on adult Human Dermal Fibroblast (HDFa). O. stamineus and C. caudatus
were toxic to cells at the concentration of 100μg/ml. The effects of the plant extracts
on Type I pro-collagen production were tested and it was found that O. stamineus, C.
hirta and C. caudatus accelerated PICP expressions when tested at the concentrations
of 50, 100, 250 and 500ng/ml. For the cytoprotection effects of plant extracts at the
concentrations of 50, 100, 250 and 500ng/ml towards the sub-lethal dose of hydrogen
peroxide at 750μg/ml, only 50ng/ml of O. stamineus showed protection against the
oxidative stress. Plant extracts were further investigated by using C. elegans as the
model in vivo organism.
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