Quah , Evan Seng Huat
(2016)
Phylogeographic Structure Of Co-Distributed, Upland Lineages Of Frogs And Snakes Across Mountain Tops In Peninsular Malaysia.
PhD thesis, Universiti Sains Malaysia.
Abstract
Kawasan pergunungan di serata dunia merupakan kawasan yang mempunyai kepelbagaian tinggi dan spesies-spesies endemik dan Semenanjung Malaysia tidak terkecuali. Struktur filogeografi tiga spesies katak gunung, Metaphrynella pollicaris, Philautus petersi dan Philautus vermiculatus dan tiga genus ular yang kebanyakannya dari kawasan pergunungan, Macrocalamus, Calamaria dan Popeia dibandingkan untuk mengenalpasti hubungan populasi allopatrik. Kerja lapangan dijalankan di beberapa lokasi tanah tinggi di seluruh semenanjung dan spesimen-spesimen dikumpul untuk perbandingan genetik. Tindakbalas rantaian polymerase (PCR) dijalankan untuk mengamplifikasi gen-gen mitokondria ND1 atau CytB, jujukan-jujukan gen disusun, dianalisis dengan analisis Bayesian dan Kebarangkalian Maksimum dan dimasukan dalam analysis integratif taxonomi bersama corak warna dan morphologi.
Montane regions around the world are areas of high diversity and endemism and Peninsular Malaysia is no different. The phylogeographic structure of three species of montane frogs, Metaphrynella pollicaris, Philautus petersi and Philautus vermiculatus and three genera of largely montane snakes, Macrocalamus, Calamaria and Popeia were compared to ascertain the relationships of the different allopatric populations. Field work was conducted at various montane sites across the country and specimens were collected for genetic comparison. Following PCR amplification for the ND1 or CytB mitochondrial genes, sequences were aligned, analysed using Bayesian and Maximum likelihood analyses and included in an integrative taxonomic analysis using colour pattern and morphology.
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