Protoplast Cultures Of Oryza Sativa L. And Brachiaria Decumbens

Daud, Zainah (2015) Protoplast Cultures Of Oryza Sativa L. And Brachiaria Decumbens. PhD thesis, Universiti Sains Malaysia.

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Abstract

Sistem kultur tisu bagi padi (Oryza sativa L.) cv. Fujisaka 5 dan IRAT 13, dan Brachiaria decumbens telah berjaya dibangunkan. Teknik pensterilan dua peringkat dengan menggunakan 70 % etanol dan 20 % Clorox® adalah memadai untuk memperolehi 83.3 % hingga 100 % biji benih aseptik bergantung kepada spesis dan kultivar padi yang digunakan. Peratusan percambahan biji benih yang tinggi iaitu sebanyak 97.7 % telah dicapai untuk O. sativa cv. Fujisaka 5. Manakala O. sativa cv. IRAT 13 dan B. decumbens menunjukkan peratusan percambahan yang rendah, iaitu, masing-masing 28.0 % dan 40.0 %. Kalus O. sativa L. cv. Fujisaka 5 pada permulaannya diaruh daripada 3 jenis eksplan, biji benih matang, akar dan daun. Eksplan akar dan biji benih matang O. sativa L. cv. The tissue culture systems for the rice (Oryza sativa L.) cv. Fujisaka 5 and IRAT 13, and Brachiaria decumbens were established. Two stage surface sterilization techniques using 70 % ethanol and 20 % Clorox® was sufficient to obtain 83.3 % to 100 % aseptic seeds depending on species and rice cultivar used. A high seed germination percentage of 97.7 % was obtained for O. sativa cv. Fujisaka 5. While, O. sativa cv. IRAT 13 and B. decumbens showed low germination percentage of 28.0 % and 40.0 % respectively.

Item Type: Thesis (PhD)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: Mr Firdaus Mohamad
Date Deposited: 11 Jan 2017 00:43
Last Modified: 12 Apr 2019 05:25
URI: http://eprints.usm.my/id/eprint/31526

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