Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization

Ho, Sing Yee (2014) Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization. Masters thesis, Universiti Sains Malaysia.

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Abstract

Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage embryos. Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa panjang daripada embrio peringkat blastula ikan zebra.

Item Type: Thesis (Masters)
Subjects: G Geography. Anthropology. Recreation > G Geography (General)
S Agriculture > SF Animal culture
Divisions: Pusat Kajian Samudera & Pantai (Centre for Marine and Coastal studies (CEMACS)) > Thesis
Depositing User: Administrator Automasi
Date Deposited: 11 Jun 2015 08:13
Last Modified: 12 Apr 2019 05:26
URI: http://eprints.usm.my/id/eprint/29097

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