Md. Nasir, Siti Norfariha
(2016)
Pretreatment And Iiydrolysis Of Rice Straw And Fruits Peel Waste By Using Vermiwash For Bioethanol Production.
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
The main objective for this study was to produce ethanol from lignocellulosic
biomass which are rice straw and fruit waste peel (equal ratio of papaya, pineapple and
guava). Vermiwash was used in the hydrolysis process. The experimental works
involved were biomass characterization, bacteria isolation from venniwash and
biochemical tests such as (carboxymethylcellulase) CMC test, starch test and phenol red
test, including bacterial identification using 16S rDNA. Besides ethanol concentration,
reducing sugar and enzymatic activity determination by dinitrosalicylic acid (DNS)
method were also carried out. The study involved the screening part and also the
optimization for the factors involved in the ethanol yield using Response Surface
Methodology (RSM) and Analysis of Variance (ANOVA). Four
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