Abobaker, Mahmod Sidati Ali
(2023)
Treatment Of Leachate By
Scenedesmus Sp. Via Different Modestypes
Of Bioreactors And Its Potential
For Bioethanol Production.
PhD thesis, Universiti Sains Malaysia.
Abstract
In order to address human needs while safeguarding the environment, the
utilization of sustainable material sources is imperative, this study focuses on the
investigation of Scenedesmus sp. cultivation with varying concentrations of
polysaccharide (starch), followed by its application in leachate treatment. The main
objectives of this study were to determine the optimal concentration of
polysaccharide for Scenedesmus sp. growth, evaluate its efficiency in treating
municipal landfill leachate using batch, continuous, and membrane cultivation
modes, and explore its capacity for bioethanol production. The methodology
involved several stages, including the cultivation of Scenedesmus sp. with different
concentrations of polysaccharide to identify the ideal growth condition.
Subsequently, batch cultivation was conducted with varying pH levels, while
continuous cultivation involved different dilutions. Membrane cultivation was
carried out at various flow rates. The resulting algal biomass was then subjected to
fermentation, with and without sonication, at different pH values to produce
bioethanol. The results indicated that an optimal concentration of 0.8 g of
polysaccharide yielded the highest growth of Scenedesmus sp. In batch cultivation, a
pH of 7.0 exhibited the most effective removal percentages for COD, nitrogen,
phosphorus, and carbohydrates. In continuous cultivation, the highest removal of
COD, carbohydrate, phosphorus, and nitrogen occurred at a dilution rate of 0.220 h-1,
accompanied by the highest specific rate of COD uptake.
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