Muhammad Redzwan, Sidik
(2008)
Investigation of yb-1 gene expression by syber-green based
real-time pcr in brain tumor.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Cancer generally is a genetic disease that can cause alteration in specific gene in which a
group of cells display uncontrolled growth, invasion and metastasis. YB-1 Gene Y -box
binding protein (YB-1) is a member of the cold-shock domain (CSD) protein superfamily
and involved in many cellular functions, including transcriptional regulation, translational
regulation, DNA repair, drug resistance and stress responses to extracellular signals. YB-1
gene can be found in a cytoplasm and nuclear cell. The YB-1 gene comprises 8 exons
spanning 19 kb of genomic DNA and is located on chromosome I p34. The aim of the
study is to differential relatively quantitative the expression of YB-I gene in brain tumor
sample (meningioma) and normal brain sample (Astrocyte SVG I2). SYBR-Green I
based Real-Time PCR was performed and data analysis using formula 2-MCt has
demonstrated that YB-1 gene was over-expressed by 2.86 fold in comparison to its
expression in normal sample. This preliminary data is very important in the future
experiment for expression of YB-I gene showed the potential as a biology marker for
detection of brain tumor.
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