The effect of diet and BMI on gut microbiota profile among primary school children in Kota Bharu, Kelantan

Muhammad, Nur Amalina (2018) The effect of diet and BMI on gut microbiota profile among primary school children in Kota Bharu, Kelantan. Masters thesis, Universiti Sains Malaysia.

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Human gut microbes are present in large concentration on intestinal mucosal surfaces and play important roles in host health. Obesity and dietary intake are known to have implications in driving factors for structure of gut microbiota. Method The present study examined the composition of the gut microbial community among primary school children in Kota Bharu, aged 7-11 years old (n=81). Fecal sample were collected and subjected to 16S rRNA gene sequencing analysis. Dietary survey were obtained in order to assessed the association of diet between gut microbiota. Result Analysis of bacterial composition according to taxonomic rank (genus, family and phylum) revealed most abundance of bacterial at genus level were Bacteroides 23% and Prevotella 22 % respectively. The microbiota were classified into two enterotype like clusters , each driven by Bacteroides (B-Type) or Prevotella (P-Type). Statistical analysis revealed B-Type and PType shows significant association with BMI (p value =0.005).We also found that B-Type of microbiota positively associated with chicken and fish (p value = 0.007 and 0.038 respectively), whereas P-Type showed positively associated with fruit, milk & dairy product, seafood, seasoning & flavourings and beverage (p value = 0.025, 0.020, 0.032, 0.001 and 0.012 respectively). Conclusion There was an association between BMI and diet on gut microbiota among healthy primary school children in the Kota Bharu population. Further studies are necessary to elucidate the mechanism behind these changes and ultimately their link to health and disease.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Gastrointestinal microbiome
Subjects: R Medicine > R Medicine (General)
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Thesis
Depositing User: Mr Abdul Hadi Mohammad
Date Deposited: 01 Sep 2020 02:40
Last Modified: 01 Sep 2020 02:40

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