Alif Faiz Mohd Din, Muhammad
(2020)
Extraction Of Secondary Metabolite In
Tetraselmis Suecica Using Different
Solvent Polarity.
Project Report.
Pusat Pengajian Teknologi Industri.
(Submitted)
Abstract
Recently, there has been much interest in secondary metabolites extracted from natural
sources including microalgae for the food industry as well as pharmaceutical, cosmetic,
and nutraceutical sectors. These interests have been increasing among researchers
nowadays. Therefore, the present investigation was undertaken to analyse the
secondary metabolite from Tetraselmis suecica which is a marine microalga. Briefly,
T. suecica was cultured in normal conditions in the F/2 medium. During 15 days of
monitoring of the cell growth, the absorbance reading at 680 nm was recorded. Then,
its biomass was further extract by using solvent extraction method. Further, the extract
was obtained using different solvent polarity which are hexane, ethyl acetate, and
methanol. The yield extract was calculated and was revealed that extraction using
hexane as solvent was the best among those three solvents. The resulted extract using
different solvent polarities was further analyse using GC-FID. There were several peaks
present in the chromatogram of extract using the solvents tested, however, there is no
peak in the chromatogram of extract using methanol. This can be concluded that there
was a compound which is C10 (Methyl Decanoate) in the chromatogram of extract
using hexane and several compounds in the chromatogram of extract using ethyl acetate
such as C10 (Methyl Decanoate), C12 (Methyl Dodecanoate) and C14 (Methyl-Cis-9-
Tetradecanoate)
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