Yan Leong, Kah
(2020)
Physical Extraction Of Flagellavia
Ultrasonication From Bacillus
Subtilis 168 With Various
Control Elements.
Project Report.
Pusat Pengajian Teknologi Industri.
(Submitted)
Abstract
Flagella was commonly known as the tail of bacteria. The major structural protein of
bacterial flagella is called flagellin. Flagella have a potential to become biodegradable
nanotube and important for drug delivery, electronic or semiconductor. There are still
lack of research about flagella separation and purification. In this research, the
morphology of the flagella and basal body for Bacillus subtilis 168 was investigated
by scanning electron microscope and transmission electron microscope. The
characterization of flagella was found that difference between flagella and cell body is
nitrogen (N), magnesium (Mg) and Sulphur (S) by energy-dispersive X-ray
spectroscopy. Statistically designed experiment revealed significant effects of
sonication duration and sonicator output power on the concentration of flagellin after
sonication. The cell body of Bacillus subtilis 168 was started to breaked from 20 %
sonicator output power.
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