Wen Chian, Liew
(2020)
Microbial Production Of
Polyhydroxybutyrate (Phb) By Burkholderia
Cepacia Bpt1213 From Different Carbon
Sources And Its Fermentation Kinetics.
Project Report.
Pusat Pengajian Teknologi Industri.
(Submitted)
Abstract
The issue of plastic pollution has become a major threat to global ecology due
to plastic’s resilience against degradation and its proliferation in industry. As a result,
the biodegradable polymer such as polyhydroxyalkanoate (PHA) is considered as a
good substitute to the conventional petroleum-based polymers because of their similar
material properties to synthetic polymers and complete biodegradability after disposal.
However, PHA homopolymer, poly-3-hydroxybutyrate (P(3HB)) is the most common
and widely studied PHA compounds. In this study, the biosynthesis of P(3HB)
polymer produced by Burkholderia cepacia BPT1213 using different carbon source
such as banana trunk juices, molasses and oil palm frond juices was studied. Besides,
the sugar contents (sucrose, glucose and fructose) of these carbon sources were
analysed. Molasses has the highest sucrose, glucose and fructose content (8.41 ± 0.50
mg/mL, 35.34 ± 0.01 mg/mL and 33.88 ± 0.06 mg/mL respectively). Next, kinetic
study of B. cepacia BPT1213 such as specific growth rate, doubling time, biomass and
PHA productivity were investigated for greater understanding of the fermentation
efficiency.
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