Pauline, Liew Woan Ying
(2013)
Involvement Of Azotobacter Vinelandii
Hypothetical Protein Avin_16040 In Oryza
Sativa Root Attachment.
PhD thesis, Universiti Sains Malaysia.
Abstract
In this study, the dynamic changes of Azotobacter vinelandii ATCC 12837's
proteome in response to Oryza sativa L. cv. MR 219, a local rice variety, was
observed. Analysis by 2DE MS/MS revealed various protein spots which showed
differential presence when A. vinelandii ATCC 12837 was grown in the N-enriched
(+N) or N-free (-N) media. Similar experimental setup was also applied to study the
profiles when the bacterial strain was exposed to root. The differentially-present
intracellular proteins were identified by MALDI-TOF/TOF analysis and these
included those involved in metal ion transport and storage, respiration, stress
response, structure, regulatory, amino acid synthesis, and electron transport. The
identified differentiated extracellular proteins were membrane protein transporters,
lipoprotein and oxygen stress response proteins. Besides that, several hypothetical
proteins with unknown function were also differentiated. Some of these bacterial
proteins demonstrated induced expression under N deficient conditions. There are
also a number of other proteins that showed differential presence when A. vinelandii
ATCC 12837 was exposed to rice roots compared to those unexposed. The
hypothetical protein Avin_16040, which showed exclusive presence when A.
vinelandii ATCC 12837 colonized root surface, was further analyzed. To investigate
the biological function of Avin_16040, a deletion mutant of the Avin_16040 gene
was generated by homologous recombination.
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