Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids

Ishak, Rafidah (2012) Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids. Masters thesis, Universiti Sains Malaysia.

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Abstract

Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be induced from the leaf, petiole and root explants of the in vitro plantlets on MS medium supplemented with 2.0 - 10.0 mg/L picloram. The leaf explants inoculated to MS medium supplemented with 2.0 mg/L picloram produced the best callus for cell suspension cultures.

Item Type:	Thesis (Masters)
Subjects:	Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions:	Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User:	ASM Ab Shukor Mustapa
Date Deposited:	05 Jul 2019 07:42
Last Modified:	05 Jul 2019 07:42
URI:	http://eprints.usm.my/id/eprint/44885

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