Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum

Shim , Siang yian (2010) Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum. Masters thesis, Universiti Sains Malaysia.

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Abstract

A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein.

Item Type:	Thesis (Masters)
Subjects:	T Technology > TP Chemical Technology > TP1-1185 Chemical technology
Divisions:	Kampus Kejuruteraan (Engineering Campus) > Pusat Pengajian Kejuruteraan Kimia (School of Chemical Engineering) > Thesis
Depositing User:	ASM Ab Shukor Mustapa
Date Deposited:	17 Oct 2018 08:39
Last Modified:	12 Apr 2019 05:26
URI:	http://eprints.usm.my/id/eprint/42578

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