Rashid, Syarifah Ab
(2009)
Pengoptimuman, Penulenan Dan Pencirian
Enzim Mannanase Daripada Aspergillus Niger
Usm F4 Melalui Pemfermentasian Substrat
Pepejal (Ssf) Dengan Menggunakan Isirong
Kelapa Sawit Sebagai Substrat.
Masters thesis, Universiti Sains Malaysia.
Abstract
Daripada 9 pencilan kulat yang disaring, didapati pencilan USM F4 mampu
menghasilkan aktiviti mannanase yang lebih tinggi berbanding dengan 8 pencilan lain.
Pengecaman melalui morfologi dan mikroskopik menunjukkan kulat tersebut mempunyai
ciri-ciri Aspergillus niger. Penghasilan mannanase oleh A. niger USM F4 dioptimumkan
berdasarkan keadaan pengkulturan dan medium pemfermentasian dalam dua sistem
utama; sistem kelalang goncangan dan dulang.
From the 9 isolates of fungi that were screened, the isolate USM F4 was found capable to
produce higher mannanase activity than the other 8 of fungal isolates. The colony and
microscopic morphologies identification showed that this fungal was very identical to
Aspergillus niger. The production of mannanase by A. niger USM F4 was futher
optimised based on the cultural conditions and fermentation medium in two main
systems; flask and tray systems.
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