Zulkipli, Anis Fadhilah
(2016)
Apoptosis Activity and Mitogen Activated Protein Kinase
Expression of the Mouse Macrophage Cell Line J774a.1
Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1
of Plasmodium Falciparum.
Masters thesis, USM.
Abstract
Apoptosis makrofaj merupakan mekanisme yang berkesan dalam mengawal
jangkitan intrasel semasa gerak balas imun semulajadi terhadap pelbagai patogen
termasuk parasit malaria. Kajian ini dijalankan untuk menentukan aktiviti apoptosis
dan pengekspresan protein pengaktifan mitogen (MAPK) dalam sel makrofaj mencit
J774A.1 yang dijangkiti klon BCG dan BCG rekombinan (rBCG) yang
mengekspreskan terminus C protein permukaan merozoite-1 (MSP-1C) daripada
Plasmodium falciparum selama 48 jam. Pewarnaan nukleus menggunakan Hoest
33342 menunjukkan bahawa klon rBCG berupaya meningkatkan kondensasi nuklear
dan peringkat morfologi apoptosis dalam sel makrofaj yang dijangkiti berbanding
BCG dan LPS.
Macrophage apoptosis exerts an efficient mechanism in controlling intracellular
infection during innate immune response against various pathogens including malaria
parasites. This study was carried out to determine the apoptosis activity and mitogen
activated protein kinase (MAPK) expression in mouse macrophage cell line J774A.1
infected with a Mycobacterium bovis bacille Calmette-Guerin (BCG) clone and a
recombinant BCG (rBCG) clone expressing the C-terminus of merozoite surface
protein-1 (MSP-1C) of Plasmodium falciparum for 48 hours. The nuclear staining
with Hoechst 33342 showed that the rBCG clone was capable of increasing the
nuclear condensation and morphological stages of apoptosis in the infected cells
compared to the BCG-infected cells and the LPS-stimulated cells. The flow
cytometric analysis using Annexin-V and PI staining confirmed that the rBCG clone
significantly increased the percentage of early apoptotic activity in the infected
macrophage higher than the one stimulated by the parent BCG clone and LPS.
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