Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

Omar, Noorsharmimi and Loh, Qiuting and Gee, Jun Tye and Yee, Siew Choong and Noordin, Rahmah and Jörn, Glökler and Lim, Theam Soon (2014) Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors, 14 (1). pp. 346-355. ISSN 1424-8220

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Abstract

G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.

Item Type:	Article
Subjects:	R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions:	Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Article
Depositing User:	Mr Noorazilan Noordin
Date Deposited:	02 Feb 2018 09:20
Last Modified:	02 Feb 2018 09:20
URI:	http://eprints.usm.my/id/eprint/38675

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