Amelioration of Isoproterenol-Induced Oxidative Damage in Rat Myocardium by Withania somnifera Leaf Extract

Khalil, Md. Ibrahim and Ahmmed, Istiyak and Ahmed, Romana and E. M., Tanvir and Afroz, Rizwana and Paul, Sudip and Siew, Hua Gan and Alam, Nadia (2015) Amelioration of Isoproterenol-Induced Oxidative Damage in Rat Myocardium by Withania somnifera Leaf Extract. BioMed Research International, 2015 (624159). pp. 1-10. ISSN 2314-6133

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We investigated the protective role of Withania somnifera leaf extract (WSLEt) on isoproterenol- (ISO-) induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (85mg/kg body weight (b.w.)) administered to rats for two consecutive days caused a significant increase in cardiac troponin I (cTnI) levels and serum lipid profiles, as well as the activities of some marker enzymes. In addition to these diagnosticmarkers, there were increased levels of lipid peroxidation (LPO) and decreased activities of enzymatic antioxidants (superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRx), and glutathione- S-transferase (GST)) in themyocardium.However, oral pretreatment (100mg/kg b.w.) withWSLEt for 4 weeks elicited a significant cardioprotective activity by lowering the levels of cTnI, lipid profiles, and marker enzymes. The levels of LPO products were also significantly decreased. Elevated activities of antioxidant enzymes were also observed in rats pretreated with WSLEt. As further confirmed histopathologically, our findings strongly suggest that the cardioprotective effect ofWSLEt onmyocardium experiencing ISO-induced oxidative damage may be due to an augmentation of the endogenous antioxidant system and an inhibition of LPO in the myocardial membrane.We conclude thatWSLEt confers some protection against oxidative damage in ISO-inducedMI in rats.

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Article
Depositing User: Mr Noorazilan Noordin
Date Deposited: 08 Jan 2018 07:09
Last Modified: 08 Jan 2018 07:09

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