Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats

H, Hermizi and O, Faizah and S Ima, Nirwana and S Ahmad, Nazrun and DA , Luke and M, Norazlina (2007) Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats. Annals of Microscopy , 7. pp. 10-24. ISSN 0219-2209

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The effects of nicotine administration on structural and cellular parameters of bone histomorphometry, cotinine, and biomarkers of bone remodeling were studied in twenty-one Sprague-Dawley male rats. Rats aged three months and weighing between 250-300 g were divided into three groups. Group 1 was the baseline group, which was sacrificed without treatment. The other 2 groups were the control group and the nicotine group. The nicotine group was treated with nicotine 7 mg/kg body weight and the C group was treated with normal saline only. Treatment was given by intraperitoneal injection, six days a week for a period of 4 months. Histomorphometric analysis was done on the metaphyseal region of the trabecular bone of the left femur by using an image analyzer. Biochemical analysis was done using ELISA-test kit to compare the serum cotinine, osteocalcin and pyridinoline (PYD) levels between pretreatment and after 4 months treatment in the control and nicotine groups. Histomorphometric analysis revealed that nicotine significantly decreased the trabecular bone volume (BV/TV) and the osteoblast surface (Ob.S/BS), and increased the osteoclast surface (Oc.S/BS) and the eroded surface (ES/BS) compared to the baseline and control groups. In addition, biochemical analysis showed that nicotine treatment for 4 months significantly decreased the osteocalcin (bone formation marker) levels while the cotinine and PYD (bone resorption marker) levels were increased as compared to pretreatment. We concluded that treatment with nicotine 7 mg/kg for 4 months exerted negative a effect on the trabecular bone histomorphometric parameters and bone remodeling biomarkers.

Item Type: Article
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Article
Depositing User: Mr Noorazilan Noordin
Date Deposited: 26 Jul 2017 00:30
Last Modified: 26 Jul 2017 00:30

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