H, Hermizi and O, Faizah and S Ima, Nirwana and S Ahmad, Nazrun and DA , Luke and M, Norazlina
(2007)
Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats.
Annals of Microscopy , 7.
pp. 10-24.
ISSN 0219-2209
Abstract
The effects of nicotine administration on structural and cellular parameters of bone
histomorphometry, cotinine, and biomarkers of bone remodeling were studied in twenty-one
Sprague-Dawley male rats. Rats aged three months and weighing between 250-300 g were divided
into three groups. Group 1 was the baseline group, which was sacrificed without treatment. The
other 2 groups were the control group and the nicotine group. The nicotine group was treated with
nicotine 7 mg/kg body weight and the C group was treated with normal saline only. Treatment was
given by intraperitoneal injection, six days a week for a period of 4 months. Histomorphometric
analysis was done on the metaphyseal region of the trabecular bone of the left femur by using
an image analyzer. Biochemical analysis was done using ELISA-test kit to compare the serum
cotinine, osteocalcin and pyridinoline (PYD) levels between pretreatment and after 4 months
treatment in the control and nicotine groups. Histomorphometric analysis revealed that nicotine
significantly decreased the trabecular bone volume (BV/TV) and the osteoblast surface (Ob.S/BS),
and increased the osteoclast surface (Oc.S/BS) and the eroded surface (ES/BS) compared to the
baseline and control groups. In addition, biochemical analysis showed that nicotine treatment
for 4 months significantly decreased the osteocalcin (bone formation marker) levels while the
cotinine and PYD (bone resorption marker) levels were increased as compared to pretreatment.
We concluded that treatment with nicotine 7 mg/kg for 4 months exerted negative a effect on the
trabecular bone histomorphometric parameters and bone remodeling biomarkers.
Actions (login required)
 |
View Item |
![[img]](http://eprints.usm.my/style/images/fileicons/application_pdf.png)
