Mohamed , Norazlina and Hapidin, Hermizi and Othman , Faizah and Ahmad Shuid, Nazrun and Muhammad , Norliza and Soelaiman , Ima-Nirwana
(2010)
Vitamin E reversed nicotine-induced toxic effects on bone
biochemical markers in male rats.
Arch Med Sci, 6 (4).
pp. 505-512.
Abstract
Introduction: Vitamin E is beneficial in restoring bone histomorphometric
parameters in nicotine-treated rats. This study determined the effectiveness of
3 forms of vitamin E in restoring bone metabolism in nicotine-treated rats.
Material and methods: Thirty-five male Sprague-Dawley rats were divided into
5 groups: (1) control (C), (2) nicotine cessation (NC), (3) α-tocopherol (ATF), (4)
tocotrienol-enhanced fraction (TEF) and (5) γ-tocotrienol (GTT). Treatment was
carried out for 4 months. The control group was administered normal saline and
olive oil throughout the treatment period while treatment for groups 2-5 was
performed in 2 phases. In the first phase, the groups received nicotine 7 mg/kg
intraperitoneally for 2 months. The following 2 months, group 2 received normal
saline and olive oil while groups 3-5 received ATF, TEF or GTT, 60 mg/kg orally.
Pre-treatment and post-treatment serum was collected for bone biochemical
marker measurement using the ELISA method.
Results: Nicotine increased serum bone-resorbing cytokines (interleukin-1 and
interleukin-6) and the bone resorption marker pyridinoline (PYD) while reducing
the bone formation marker osteocalcin after 2 months of nicotine treatment.
The parameters failed to improve after nicotine was stopped for 2 months.
Supplementation with the 3 forms of vitamin E improved the parameters, i.e.
reduced the cytokines and pyridinoline as well as increased the osteocalcin. In
addition, the TEF and GTT groups had a higher level of osteocalcin than the
control group.
Conclusions: Nicotine impaired bone metabolism and cessation of nicotine
treatment did not reverse the effects. Vitamin E, especially the tocotrienols,
restored bone metabolism that was impaired due to nicotine.
Actions (login required)
 |
View Item |
![[img]](http://eprints.usm.my/style/images/fileicons/application_pdf.png)
