Yong, Cheng Chung
(2016)
Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus.
PhD thesis, Universiti Sains Malaysia.
Abstract
Tiga puluh enam strain bakteria asid laktik telah diasingkan daripada tenusu, daging dan produk penapaian tempatan. Ekstrak extrasel (CFS) daripada L. plantarum USM8613, yang didapati daripada sosej yang ditapai, menunjukkan aktiviti perencatan dan penghasilan asid laktik yang lebih tinggi (ρ < 0.05) telah dipilih untuk analisis seterusnya. Kajian ex-vivo menunjukkan CFS daripada L. plantarum USM8613 berupaya merencatkan pembentukan biofilem dan pertumbuhan S. aureus pada kulit khinzir. Fraksi yang kaya dengan protein, lemak dan polisakarida yang diekstrak daripada CFS L. plantarum USM8613 telah diuji untuk aktiviti perencatan. Kajian menunjukkan fraksi yang kaya dengan protein memberi kesan yang lebih ketara berbanding dengan fraksi yang kaya lemak and polisakarida. Fraksi yang kaya dengan protein dari L. plantarum USM8613 juga berupaya merencatkan (ρ <0.05) pertumbuhan S. aureus klinikal dan pembentukan biofilem pada kulit khinzir. Rawatan topikal yang mengandungi 800 AU/mL fraksi yang kaya dengna protein daripada L. plantarum USM8613 didapati berupaya mengurangkan (ρ < 0.05) bilangan sel S. aureus ditapak luka tikus.
Thirty-six strains of lactic acid bacteria were isolated from local dairy, meat and fermented products. Cell-free-supernatant (CFS) of L. plantarum USM8613, isolated from fermented sausage, was selected for subsequent analyses. The CFS exhibited a significantly stronger (ρ < 0.05) inhibitory activity against S. aureus and produced a higher amount of lactic acid as compared to all strains studied. Ex-vivo study demonstrated CFS from L. plantarum USM8613 inhibited the growth and biofilm formation of S. aureus on porcine skins. CFS of L. plantarum USM8613 was fractionated into protein-rich, lipid-rich and polysaccharide-rich fractions, and all fractions exhibited significant inhibitory activity, with a more prevalent effect from the protein-rich fraction. The antimicrobial and anti-biofilm effects of the protein-rich fraction were further confirmed with S. aureus-infected porcine skins. Topical application of ointment containing 800 AU/mL of the protein-rich fraction from L. plantarum USM8613 significantly reduced (ρ < 0.05) the cell counts of S. aureus in the wound site of S. aureus infected-rats.
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