Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue

Othman, Ekhlas Qaid Gazem (2016) Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue. PhD thesis, Universiti Sains Malaysia.

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    Abstract

    Pengesanan beberapa jenis kanser boleh dilakukan dengan menggunakan antibodi monoklon (AbM). Kajian menunjukkan bahawa jenis-jenis kanser yang berbeza mempunyai protein tak normal yang tertentu yang boleh dikenal pasti oleh AbM. IBMR3 AbM dibangunkan daripada peptida sintetik yang berhubung dengan jujukan asid amino terpilih daripada reseptor interleukin-4 manusia. Kajian ini adalah untuk mengesan ekspresi IBMR3 antigen dalam karsinoma termasuk Manusia carcinoma berhubung dgn pangkal tekak epitelium (HEp-2), adenokarsinoma kolon Manusia (HT-29) dan tisu kanser payudara. Ungkapan IBMR3 antigen dikesan pada HEp-2 dan HT-29 menggunakan ujian berbentuk tidak langsung pewarnaan, aliran sitometri pewarnaan. Hasil kajian menunjukkan persatuan kemungkinan IBMR3 antigen ungkapan dalam karsinoma (kanser sel-sel epitelium) dengan pewarnaan cytoplasmic positif. HEp-2 dan HT-29 telah dipisahkan melalui 1-D immunoblot dan keputusan menunjukkan dua band dengan berat molekul (MWS) 45 dan 25 kDa dalam HT-29 dan satu band dengan MWS 25 kDa dalam HEp-2. IBMR3 antigen ungkapan itu dikesan pada 48 sampel kanser payudara Malaysia dan microarray (TMA) slaid tisu kanser payudara 430 dan 132 tisu payudara yang normal bersebelahan dengan kanser payudara melalui IHC. The detection of several types of cancer is possible by using monoclonal antibody (MAb). Studies have shown that different types of cancer have related specific abnormal proteins, which are identified by MAb. IBMR3 is a MAb which was raised against synthetic peptides that are associated with the selected amino acid sequence of human interleukin-4 receptors. This research is to detect the expression of IBMR3 antigen in carcinoma including Human laryngeal epithelial carcinoma (HEp-2), Human colon adenocarcinoma (HT-29) and breast cancer tissues. The expression of IBMR3 antigen was detected on HEp-2 and HT-29 using indirect immunofluorescent staining, flow cytometry staining. The results showed the possible association of IBMR3 antigen expression in carcinoma (epithelial cancer cells) with positive cytoplasmic staining. HEp-2 and HT-29 were separated via 1-D immunoblot and the results showed 2 bands with molecular weights (MWs) of 45 and 25 kDa in HT-29 and one band with MWs of 25 kDa in HEp-2. IBMR3 antigen expression was detected on 48 samples of Malaysian breast cancer and microarray (TMA) slides of 430 breast cancer tissues and 132 normal breast tissues adjacent to breast cancer through IHC.

    Item Type: Thesis (PhD)
    Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
    Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 20 Feb 2017 15:55
    Last Modified: 10 Apr 2017 10:12
    URI: http://eprints.usm.my/id/eprint/32143

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