Yong, Ya Fen (2025) Regioselective Biosynthesis Of Reveromycin Derivatives By Cytochrome P450revi Mutant In Actinacidiphila Reveromycinica Sn-593. PhD thesis, Universiti Sains Malaysia.
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Abstract
Reveromycin A (RM-A) (1) has a 6,6-spiroacetal core structure that is important for its biological activity. However, 1 undergoes a spiroacetal rearrangement to form RM-B (2) with a 5,6-spiroacetal core, which exhibits reduced bioactivity. This undesired rearrangement is partly due to the hemisuccinate moiety at the C18 position of 1. In 1 biosynthesis, P450revI catalyzes the C18-hydroxylation of RM-T (3), which is essential for its subsequent hemisuccinylation to generate 1. This study aimed to alter the P450revI regioselectivity to improve the stability of the 6,6-spiroacetal core and expand the structural diversity of RMs.
| Item Type: | Thesis (PhD) |
|---|---|
| Subjects: | R Medicine > R Medicine (General) > R5-920 Medicine (General) |
| Divisions: | Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Thesis |
| Depositing User: | Mr Aizat Asmawi Abdul Rahim |
| Date Deposited: | 02 Sep 2026 01:35 |
| Last Modified: | 02 Sep 2026 01:35 |
| URI: | http://eprints.usm.my/id/eprint/64867 |
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