Hussin Khan, Ibrahim (2017) Assessment Of Proliferation Rate In Breast Cancer By Ki-67 Labeling Index. Masters thesis, Universiti Sains Malaysia.
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Abstract
Introduction: Breast cancer is the commonest cancer in women. One of the characteristics of breast cancer is its association with the limitless replicative potential of the malignant cells. This cellular proliferation can be gauged by looking at Ki-67 labelling index, which is a type of proliferation biomarker. This study aims to compare the Ki-67 labelling index between the manual and the automated counting methods with various cut-off values. Methods: Eighty-eight breast cancers that met the inclusion criteria from year 2014 to 2016 were recruited in this study. Clinicopathological data were extracted from the pathology reports. Immunohistochemistry for Ki-67 was performed on tissue sections. Manual counting and automated counting using ImmunoRatio software were performed to assess the Ki-67 labelling index. Two different cut-off points, i.e. <14% and <20% were addressed. Results: Most of the patients diagnosed were more than 40 years old and majority were Malays. Ki-67 labelling index by manual counting correlated with automated method (r=0.99; p<0.001). When shifting the cut-off value from <14% to <20%, as compared to manual count, automated method resulted in a larger number of Luminal B breast cancers re-classified into Luminal A breast cancers. Using <20% as cut-off point, 3 cases that were assessed as >20% by manual counting was downgraded as <20% by automated method. Conclusion: The automated method is reliable in assessing proliferation rate as compared to the manual method, although a minority of cases showed discrepancy between these two method
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine |
| Divisions: | Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis |
| Depositing User: | MUHAMAD AMIN BIN AZMI |
| Date Deposited: | 03 Aug 2026 03:17 |
| Last Modified: | 03 Aug 2026 03:17 |
| URI: | http://eprints.usm.my/id/eprint/64740 |
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