Study On The Adsorption DNA On Β-cyclodextrin Coated Fe3 O4 Nanoparticles

Goh, Ying Hwa (2017) Study On The Adsorption DNA On Β-cyclodextrin Coated Fe3 O4 Nanoparticles. Masters thesis, Universiti Sains Malaysia.

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Abstract

The primary purpose of thi s study is to investigate the adsorpti on of deoxyribonucleic acid onto surface coated magnetic nanoparticles (MNP) by P-cyclodextrin. P-cyclodextrin coated magnetic nanoparticles, P-CD-MNP-TDI were used to adsorb and isolate deoxyribonucleic acid, DNA from liquid to solid phase. The amount of adsorbed DNA was measured using spectrophotometer at 258 nm. Different parameters affecting the adsorption of DNA onto nanoparticles were studied in this project to obtain the optimum adsorption condition for P-CD-MNP-TDI. Different kinetic, isoterms and thermodynamic models were used to study and described the adsorption behaviour of DNA onto nanoparticles. Surface-coated magnetic nanoparticles P-CD-MNP-TDI, in evident with study attested to work more efficient than magnetic nanoparticles with adsorption efficiency at 67%. The MNP and P-CD-MNP-TDI was characterized using fourier transform infrared (FT-lR) device and vibrating sample magnetometer (VSM). The characteristic spectra of P-CD-MNP-TDI was shown in the characteristic peak at 1657 cm-1 which is attributed by the dematerialization of isocyanate group which was originall y found on toluene-2,4-d iisocyanate (TDI) with the emergence of carbamate linkage (NHCO) at peaks 1657 cm-1 . The presence of carbamate linkage signifies the completion of polymerization between TOT and P-cyclodextrin. The magnetic moment ofP-CD-MNP-TDI (- ±40 emu/g) was lower than MNP magnetic moment(- ±60 emu/g)when both nanoparticles were subjected to the same amount of external magnetic field due to the present of the coating material which suggestively chaperone and reduced the inherently large surface energies. The optimum working parameter of ~-CD-MNP-TDI was found to be at pH 6, 298 K, with adsorption process reaching equilibrium at 20 minutes, and efficient initial DNA concentration being 10 mg/L. Ionic strength effect created by sodium chloride at range of 0 to 0.40 mol/L have minimal effect on the adsorption capacity of ~-CD-MNP-TDI. The adsorption efficiency of ~-CD-MNP-TDl was found to perform on a par of 70 ± 5 % after being recycled for three times. The adsorption isotherms and kinetics of DNA onto ~-CD-MNP-TDI was found to be best described by Freundlich isotherm, pseudo-second order kinetics. The entropy and enthalpy change of DNA on ~-CD-MNP-TDI were -1 2.1567 J/Kmol and -3.7371 J/mol respectively. The progression of Gibbs free energy from negative value to positive value indicates that the adsorption process is thermodynamically feasible, spontaneous and chemically controlled. The findings from the sorption studies suggest that the interaction between adsorbent and adsorbate occur through inclusion complex, hydrophobic interaction, hydrogen bonding and Vander Waals force.

Item Type:	Thesis (Masters)
Subjects:	R Medicine
Divisions:	Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis
Depositing User:	MUHAMAD AMIN BIN AZMI
Date Deposited:	21 Jul 2026 00:47
Last Modified:	21 Jul 2026 00:47
URI:	http://eprints.usm.my/id/eprint/64683

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