Gan, Shin Yi (2025) Characterisation Of Recombinant Igg Binding Protein, Fcγriia For Biosensor Development. PhD thesis, Universiti Sains Malaysia.
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Abstract
Antibodies play an important role in diagnostic applications due to their high specificity and affinity towards target antigens. However, in biosensor development, particularly for point-of-care (POC) diagnostics, the method of antibody immobilisation on sensor surfaces remains a critical bottleneck influencing assay sensitivity, reproducibility, and overall performance. Traditional techniques, such as passive adsorption or random covalent coupling, often result in poorly oriented and unstable antibodies. Consequently, assays showed high background noise, reduced binding activity, poor reproducibility, and relatively high detection limits, typically in the microgram-per-millilitre to high nanogram-per-millilitre range. Such shortcomings limit the reliability and clinical application of electrochemical immunosensors. To address these challenges, this study investigated the application of recombinant human IgG Fc receptors, namely FcγRIA, FcγRIIA, and the neonatal Fc receptor (FcRn), as site-specific antibody capture ligands for electrochemical biosensing.
| Item Type: | Thesis (PhD) |
|---|---|
| Subjects: | R Medicine > R Medicine (General) > R5-920 Medicine (General) |
| Divisions: | Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Thesis |
| Depositing User: | Mr Aizat Asmawi Abdul Rahim |
| Date Deposited: | 26 Jun 2026 01:58 |
| Last Modified: | 26 Jun 2026 01:58 |
| URI: | http://eprints.usm.my/id/eprint/64489 |
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